Effects of glucose on [ATP]c changes in α-cells within intact mouse islets (B–D) and in αTC1-9 cells (E–H). A: An intact mouse islet was infected with the PPG-Luc adenovirus, and bioluminescence was imaged in the presence of luciferin,
as described in research design and methods. Photon production is represented in pseudocolor (dark blue-yellow), representing the range 0–15 photon · min−1 · pixel−1 (in 20 mmol/l glucose). B–G: The concentration of glucose (G) was either increased or decreased from 0, 1, 3, or 5 to 20 mmol/l as indicated, whereas
2 mmol/l azide was applied after 15 min to inhibit complex IV of the respiratory chain, and thus mitochondrial ATP synthesis.
H: αTC1-9 cells were perifused throughout in the absence of glucose. After 5 min, 17 nmol/l insulin was applied for 10 min.
Values are means ± SE for 10–20 islets from four separate cultures or 5–16 independent experiments for αTC1-9 cells.