Glucose or Insulin, but not Zinc Ions, Inhibit Glucagon Secretion From Mouse Pancreatic α-Cells

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 1.
FIG. 1.

Effects of glucose on [ATP]c changes in α-cells within intact mouse islets (BD) and in αTC1-9 cells (EH). A: An intact mouse islet was infected with the PPG-Luc adenovirus, and bioluminescence was imaged in the presence of luciferin, as described in research design and methods. Photon production is represented in pseudocolor (dark blue-yellow), representing the range 0–15 photon · min−1 · pixel−1 (in 20 mmol/l glucose). BG: The concentration of glucose (G) was either increased or decreased from 0, 1, 3, or 5 to 20 mmol/l as indicated, whereas 2 mmol/l azide was applied after 15 min to inhibit complex IV of the respiratory chain, and thus mitochondrial ATP synthesis. H: αTC1-9 cells were perifused throughout in the absence of glucose. After 5 min, 17 nmol/l insulin was applied for 10 min. Values are means ± SE for 10–20 islets from four separate cultures or 5–16 independent experiments for αTC1-9 cells.

This Article

  1. Diabetes vol. 54 no. 6 1789-1797