Glucose or Insulin, but not Zinc Ions, Inhibit Glucagon Secretion From Mouse Pancreatic α-Cells

TABLE 1

Glucagon secretion from αTC1-9 cells

0 mmol/l glucose (ng · 30 min−1 · well−1) 20 mmol/l glucose (ng · 30 min−1 · well−1)
No additions 5.34 ± 0.30 4.15 ± 0.24 *
17 nmol/l insulin 4.42 ± 0.28 *
17 nmol/l insulin + 100 nmol/l wortmannin 5.21 ± 0.40
17 nmol/l insulin + 30 μmol/l ZnCl2 4.41 ± 0.21 *
  • Data are the means ± SE for four separate experiments. The effects of insulin or ZnCl2 were tested on glucagon secretion in either the absence or presence of 20 mmol/l glucose in αTC1-9 cells.

  • *

    * P < 0.05 vs. 0 mmol/l glucose.

This Article

  1. Diabetes vol. 54 no. 6 1789-1797