Differentiation and identification of EPCs from human umbilical cord blood MNCs in vitro. Differentiation of EPCs: when cord
blood MNCs were cultured on fibronectin, attached cells (A) and cell clusters (B) sprouted. C: After 7 days of culture, the cells showed enhanced differentiation, such as cord-like structure. D: Cells showed a cobblestone pattern when they matured. E: Identification of EPCs. When cells were isolated from culture plates on basement membrane matrix gel, tube formation was
identified in the matrigel. F: More than 95% of the isolated cells showed the uptake of DiI-acetylated LDL. FACS analyses of isolated cells were conducted
after 7 days of culture. Cells were labeled with fluorescent antibodies to CD34 (G) or CD45 (H).