Metallothionein inhibition of LPS/TNF-α–induced cytotoxicity in primary cultures of neonatal mouse cardiomyocytes. A: The cultures were exposed to a final concentration in the cultures of 30 μg/ml LPS plus 100 ng/ml TNF-α for 48 h and then
photographed under a microscope. B: The media were collected and spun at 10,000g for 5 min, and the supernatant was used for the measurement of LDH activity, shown by the OD value at 490 nm. C: The attached cells were removed by policeman for the measurement of 3-NT by Western blot method. Quantitative analysis of
3-NT contents (band density) from three samples of each treatment is presented relative to actin content. Treatment groups
that do not share the same letter were significantly different (P < 0.05).