Inhibition of Superoxide Generation and Associated Nitrosative Damage Is Involved in Metallothionein Prevention of Diabetic
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Urate and MnTMPyP inhibition of 3-NT formation in the LPS/TNF-α-treated cardiomyocytes. Primary cultures of neonatal cardiomyocytes
from wild-type mice were exposed to LPS/TNF-α for 48 h with or without cell-permeable urate or MnTMPyP. Coincubation of the
cells with urate or MnTMPyP significantly inhibited LPS/TNF-α–induced 3-NT formation (A) and also significantly abolished LPS/TNF-α–induced cytotoxicity LDH, shown by the OD value at 490 nm (B). Quantitative analysis of 3-NT contents (band density) from three samples of each treatment is presented relative to actin
content. Treatment groups that do not share the same letter were significantly different (P < 0.05).