GCCR and POMC expression in the pituitary gland remained unchanged after GCCR ASO treatment in ZDF rats. Rats were dosed with
saline (□), GCCR ASO 37.5 mg/kg (▪), or control ASO 37.5 mg/kg ([cjs2112]) twice a week for 4 weeks. Total mRNA was prepared
from the pituitary gland and analyzed for the expression of GCCR (A) and POMC (B) by RT-PCR (n = 4 per group). Data are expressed as means ± SE relative to saline-treated controls.