GCCR ASO treatment did not result in systemic GCCR antagonism. Rats were dosed with saline (□) or GCCR ASO (▪) at a dose of
50 mg/kg twice weekly for 4 weeks. A subset of animals was challenged with dexamethasone (Dexa) 72 h after the final dose.
Total mRNA was prepared from liver and analyzed for the expression of the following genes by RT-PCR (n = 4 per group). A: GCCR expression in GCCR ASO–treated rats not challenged with dexamethasone. *P < 0.05, GCCR ASO treatment vs. saline treatment. B: TAT expression after dexamethasone injection in liver and WAT. C: PEPCK expression after dexamethasone injection in liver. D: Total lymphocytes measured by counting and expressed relative to the mean percentage of lymphocytes in saline-treated rats.
*P < 0.05, dexamethasone treatment vs. saline treatment.