Fine-Mapping Gene-by-Diet Interactions on Chromosome 13 in a LG/J × SM/J Murine Model of Obesity

TABLE 5

Pitrm1 gene

Position 44 50 55 513 631 632 634 1005 1383 1384 1637 1747 2328 2610 2895
C57BL G G G C G T A G T G C A C G T
LG/J A T T T A C G A C A A G T A C
SM/J A T T C A C G G T G C A C G T
Codon 2 2 1 3 1 2 1 3 3 1 2 1 3 3 3
AA R-Q S-I G-C C-C V-T V-T N-D P-P P-P V-M T-K I-V N-N R-R P-P
Prob 1 −2 −3 9 −2 −2 1 7 7 −2 0 1 6 5 7
SIFT 0.01* 0.21 0.47
  • Position represents a base pair position from the 5′ end of the Pfkp gene. Sequence data from C57BL/6J mouse are from Ensembl Mouse Build 32. Codon signifies which codon the respective position represents, and AA signifies resulting amino acid change, if any. Prob signifies the log10 odds of amino acid changes derived from the BLOSUM62 matrix. SIFT signifies probabilities of amino acid changes having a significant deleterious effect.

  • *

    * Statistical significance at the 5% level. Amino acid changes between LG/J and SM/J mice are highlighted in bold. Amino acid changes between C57BL and LG/J + SM/J mice are in bold italics; these mutational changes result in no differences between LG/J and SM/J mice. Mutations at site 631 and 632 both contribute to the change from valine to threonine.

This Article

  1. Diabetes vol. 54 no. 6 1863-1872