MicroRNA Expression Is Required for Pancreatic Islet Cell Genesis in the Mouse

  1. Francis C. Lynn1,
  2. Peter Skewes-Cox1,
  3. Yasuhiro Kosaka1,
  4. Michael T. McManus12,
  5. Brian D. Harfe3 and
  6. Michael S. German14
  1. 1Diabetes Center, Hormone Research Institute, University of California San Francisco, San Francisco, California
  2. 2Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California
  3. 3Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida
  4. 4Department of Medicine, University of California San Francisco, San Francisco, California
  1. Address correspondence and reprint requests to Michael S. German, MD, University of California San Francisco Diabetes Center, University of California San Francisco, 513 Parnassus Ave., San Francisco, CA 94143-0534. E-mail: mgerman{at}diabetes.ucsf.edu

Abstract

OBJECTIVE—The generation of distinct cell types during the development of the pancreas depends on sequential changes in gene expression. We tested the hypothesis that microRNAs (miRNAs), which limit gene expression through posttranscriptional silencing, modulate the gene expression cascades involved in pancreas development.

RESEARCH DESIGN AND METHODS—miRNAs were cloned and sequenced from developing pancreata, and expression of a subset of these genes was tested using locked nucleic acid in situ analyses. To assess the overall contribution of miRNAs to pancreatic development, Dicer1, an enzyme required for miRNA processing, was conditionally deleted from the developing pancreas.

RESULTS—Sequencing of small RNAs identified over 125 miRNAs, including 18 novel sequences, with distinct expression domains within the developing pancreas. To test the developmental contribution of these miRNAs, we conditionally deleted the miRNA processing enzyme Dicer1 early in pancreas development. Dicer-null animals displayed gross defects in all pancreatic lineages, although the endocrine cells, and especially the insulin-producing β-cells, were most dramatically reduced. The endocrine defect was associated with an increase in the notch-signaling target Hes1 and a reduction in the formation of endocrine cell progenitors expressing the Hes1 target gene neurogenin3.

CONCLUSIONS—The expression of a unique profile of miRNAs is required during pancreas development and is necessary for β-cell formation.

Footnotes

  • Published ahead of print at http://diabetes.diabetesjournals.org on 5 September 2007. DOI: 10.2337/db07-0175.

    Additional information for this article can be found in an online appendix at http://dx.doi.org/10.2337/db07-0175.

    The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

    • Accepted August 26, 2007.
    • Received February 7, 2007.
| Table of Contents

This Article

  1. Diabetes vol. 56 no. 12 2938-2945
  1. Online-Only Appendix
  2. All Versions of this Article:
    1. db07-0175v1
    2. 56/12/2938 most recent