Endothelial Nitric Oxide Synthase Uncoupling Impairs Endothelial Progenitor Cell Mobilization and Function in Diabetes
- Thomas Thum1,
- Daniela Fraccarollo1,
- Maximilian Schultheiss1,
- Sabrina Froese1,
- Paolo Galuppo1,
- Julian D. Widder12,
- Dimitrios Tsikas3,
- Georg Ertl1 and
- Johann Bauersachs1
- 1Universität Würzburg, Universitatsklinikum, Medizinische Klinik I, Würzburg, Germany
- 2Division of Cardiology, Emory School of Medicine, Atlanta, Georgia
- 3Institute for Clinical Pharmacology, Medical School Hannover, Hannover, Germany
- Address correspondence and reprint requests to Dr. med. Thomas Thum or PD Dr. med. Johann Bauersachs, Universitatsklinikum, Medizinische Klinik I, Josef-Schneider Str. 2, 97080 Würzburg, Germany. E-mail: thum_t{at}klinik.uni-wuerzburg.de or bauersachs_j{at}medizin.uni-wuerzburg.de
Abstract
Uncoupling of the endothelial nitric oxide synthase (eNOS) resulting in superoxide anion (O2−) formation instead of nitric oxide (NO) causes diabetic endothelial dysfunction. eNOS regulates mobilization and function of endothelial progenitor cells (EPCs), key regulators of vascular repair. We postulate a role of eNOS uncoupling for reduced number and function of EPC in diabetes. EPC levels in diabetic patients were significantly reduced compared with those of control subjects. EPCs from diabetic patients produced excessive O2− and showed impaired migratory capacity compared with nondiabetic control subjects. NOS inhibition with NG-nitro-l-arginine attenuated O2− production and normalized functional capacity of EPCs from diabetic patients. Glucose-mediated EPC dysfunction was protein kinase C dependent, associated with reduced intracellular BH4 (tetrahydrobiopterin) concentrations, and reversible after exogenous BH4 treatment. Activation of NADPH oxidases played an additional but minor role in glucose-mediated EPC dysfunction. In rats with streptozotocin-induced diabetes, circulating EPCs were reduced to 39 ± 5% of controls and associated with uncoupled eNOS in bone marrow. Our results identify uncoupling of eNOS in diabetic bone marrow, glucose-treated EPCs, and EPCs from diabetic patients resulting in eNOS-mediated O2− production. Subsequent reduction of EPC levels and impairment of EPC function likely contributes to the pathogenesis of vascular disease in diabetes.
- BH4, tetrahydrobiopterin
- CFU, colony forming unit
- EBM, endothelial basal medium
- eNOS, endothelial nitric oxide synthase
- EPC, endothelial progenitor cell
- HPLC, high-performance liquid chromatography
- l-NNA, NG-nitro-l-arginine
- PBMC, peripheral blood mononuclear cell
- PKC, protein kinase C
- ROS, reactive oxygen species
Footnotes
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- Accepted December 12, 2006.
- Received May 22, 2006.
- DIABETES
