Dominant-Negative Effects of a Novel Mutated Ins2 Allele Causes Early-Onset Diabetes and Severe β-Cell Loss in Munich Ins2C95S Mutant Mice

  1. Nadja Herbach1,
  2. Birgit Rathkolb2,
  3. Elisabeth Kemter1,
  4. Lisa Pichl1,
  5. Matthias Klaften3,
  6. Martin Hrabé de Angelis3,
  7. Philippe A. Halban4,
  8. Eckhard Wolf2,
  9. Bernhard Aigner2 and
  10. Ruediger Wanke1
  1. 1Institute of Veterinary Pathology, University of Munich, Munich, Germany
  2. 2Institute of Molecular Animal Breeding/Gene Center, University of Munich, Munich, Germany
  3. 3Institute of Experimental Genetics, GSF-National Research Center for Environment and Health, Neuherberg, Germany
  4. 4Department of Genetic Medicine and Development, CMU, Geneva, Switzerland
  1. Address correspondence and reprint requests to Nadja Herbach, Institute of Veterinary Pathology, Veterinaerstr. 13, 80539 Munich, Germany. E-mail: herbach{at}patho.vetmed.uni-muenchen.de

Abstract

The novel diabetic mouse model Munich Ins2C95S was discovered within the Munich N-ethyl-N-nitrosourea mouse mutagenesis screen. These mice exhibit a T→A transversion in the insulin 2 (Ins2) gene at nucleotide position 1903 in exon 3, which leads to the amino acid exchange C95S and loss of the A6-A11 intrachain disulfide bond. From 1 month of age onwards, blood glucose levels of heterozygous Munich Ins2C95S mutant mice were significantly increased compared with controls. The fasted and postprandial serum insulin levels of the heterozygous mutants were indistinguishable from those of wild-type littermates. However, serum insulin levels after glucose challenge, pancreatic insulin content, and homeostasis model assessment (HOMA) β-cell indices of heterozygous mutants were significantly lower than those of wild-type littermates. The initial blood glucose decrease during an insulin tolerance test was lower and HOMA insulin resistance indices were significantly higher in mutant mice, indicating the development of insulin resistance in mutant mice. The total islet volume, the volume density of β-cells in the islets, and the total β-cell volume of heterozygous male mutants was significantly reduced compared with wild-type mice. Electron microscopy of the β-cells of male mutants showed virtually no secretory insulin granules, the endoplasmic reticulum was severely enlarged, and mitochondria appeared swollen. Thus, Munich Ins2C95S mutant mice are considered a valuable model to study the mechanisms of β-cell dysfunction and death during the development of diabetes.

Footnotes

  • Published ahead of print at http://diabetes.diabetesjournals.org on 15 February 2007. DOI: 10.2337/db06-0658.

  • The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

    • Accepted February 6, 2007.
    • Received May 12, 2006.
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  1. Diabetes vol. 56 no. 5 1268-1276
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