Generation of Nicotinic Acid Adenine Dinucleotide Phosphate and Cyclic ADP-Ribose by Glucagon-Like Peptide-1 Evokes Ca2+ Signal That Is Essential for Insulin Secretion in Mouse Pancreatic Islets
- Byung-Ju Kim1,
- Kwang-Hyun Park1,
- Chang-Yeol Yim2,
- Shin Takasawa3,
- Hiroshi Okamoto3,
- Mie-Jae Im1 and
- Uh-Hyun Kim1,4
- 1Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Republic of Korea
- 2Department of Internal Medicine, Chonbuk National University Medical School, Jeonju, Republic of Korea
- 3Department of Advanced Biological Sciences for Regeneration, Tohoku University Graduate School of Medicine, Sendai, Japan
- 4Institute of Cardiovascular Research, Chonbuk National University Medical School, Jeonju, Republic of Korea
- Address correspondence and reprint requests to Uh-Hyun Kim, MD, PhD, Department of Biochemistry, Chonbuk National University Medical School, Keum-am dong, Jeonju, 561-182, Republic of Korea. E-mail:
OBJECTIVE—Glucagon-like peptide-1 (GLP-1) increases intracellular Ca2+ concentrations ([Ca2+]i), resulting in insulin secretion from pancreatic β-cells. The molecular mechanism(s) of the GLP-1–mediated regulation of [Ca2+]i was investigated.
RESEARCH DESIGN AND METHODS—GLP-1–induced changes in [Ca2+]i were measured in β-cells isolated from Cd38+/+ and Cd38−/− mice. Calcium-mobilizing second messengers were identified by measuring levels of nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (ADPR), using a cyclic enzymatic assay. To locate NAADP- and cyclic ADPR–producing enzyme(s), cellular organelles were separated using the sucrose gradient method.
RESULTS—A GLP-1–induced [Ca2+]i increase showed a cooperative Ca2+ signal, i.e., an initial [Ca2+]i rise mediated by the action of NAADP that was produced in acidic organelles and a subsequent long-lasting increase of [Ca2+]i by the action of cyclic ADPR that was produced in plasma membranes and secretory granules. GLP-1 sequentially stimulated production of NAADP and cyclic ADPR in the organelles through protein kinase A and cAMP-regulated guanine nucleotide exchange factor II. Furthermore, the results showed that NAADP production from acidic organelles governed overall Ca2+ signals, including insulin secretion by GLP-1, and that in addition to CD38, enzymes capable of synthesizing NAADP and/or cyclic ADPR were present in β-cells. These observations were supported by the study with Cd38−/− β-cells, demonstrating production of NAADP, cyclic ADPR, and Ca2+ signal with normal insulin secretion stimulated by GLP-1.
CONCLUSIONS—Our findings demonstrate that the GLP-1–mediated Ca2+ signal for insulin secretion in pancreatic β-cells is a cooperative action of NAADP and cyclic ADPR spatiotemporally formed by multiple enzymes.
- ADPR, ADP-ribosyl
- Epac, cAMP-regulated guanine nucleotide exchange factor II
- ER, endoplasmic reticulum
- GLP-1, glucagons-like peptide-1
- GPN, glycylphenylalanine 2-naphthylamide
- KRBB, Krebs-Ringer bicarbonate buffer
- NAADP, nicotinic acid adenine dinucleotide phosphate
- PKA, protein kinase A
- Received April 2, 2007.
- Accepted January 2, 2008.