Antibiotic treatment–associated changes in gut microbiota, intestinal permeability, and endotoxemia during high-fat feeding.
A: DGGE profiles generated from the cecal microbiota in mice fed normal diet (CT), normal diet and antibiotics (CT-Ab), high-fat
diet (HF), or high-fat diet and antibiotics (HF-Ab) for 4 weeks. Each number and profile corresponds to a different animal.
Bar = Dice's similarity coefficient. B: Plasma endotoxin (LPS) concentration (EU/ml). Data are means ± SE. Data with different superscript letters are significantly
different (P < 0.05), according to the post hoc ANOVA statistical analysis. C: Intestinal permeability assay: Plasma DX-4000-FITC (μg/ml). n.d., not detectable concentration. D and F: Epithelial tight junction proteins markers (ZO-1 and occludin mRNA concentrations). E and G: Correlations between intestinal permeability markers: plasma DX-4000-FITC and epithelial tight junction ZO-1 and occludin
mRNA concentrations (P < 0.05). Inset corresponds to Pearson's r correlation and corresponding P value. Data are means ± SE. Data with different superscript letters are significantly different (P < 0.05) according to the post hoc ANOVA statistical analysis.