Mitochondrial MG-H1 immunoreactivity in C. elegans. Mitochondria were stained with MitoTracker Deep Red FM (A, D, G, and J), and MG-H1 was visualized by immunostaining with a Texas Red-labeled antibody directed against MG-H1 antibody (B, E, H, and K). Merged staining is shown in C, F, I, and L. Orange color indicates colocalization of MG-H1 with mitochondria. Images are shown for 15-day-old wild-type animals cultured
under standard (A–C) and high glucose conditions (D–F) and 15-day-old transgenic glyoxalase-1–overexpressing animals cultured under standard (G–I) and high glucose conditions (J–L). Shown are animals from a representative experiment out of three independent experiments, each including 100 nematodes.