Quantification of MG-H1 and ROS in C. elegans. Formation of MG-H1 (A) and ROS (B) was quantified in 15-day-old wild-type (WT) animals, without and with 10 μmol/l myxothiazol (myxo) or 50 μmol/l FCCP, and
in 15-day-old transgenic glyoxalase-1–overexpressing animals. Each group was cultured under standard and high glucose conditions.
The data were compared across the groups using ANOVA; additional between-group comparisons were made using Fisher PLSD post
hoc tests. Results are the means ± SE of three independent experiments with 100 nematodes each; value from a Fisher PLSD post
hoc test (*P < 0.05 and ***P < 0.001) comparing the indicated group vs. wild type under standard conditions; value from a Fisher PLSD post hoc test (oooP < 0.001) comparing the indicated group vs. wild type under high glucose conditions.