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C. elegans as Model for the Study of High Glucose– Mediated Life Span Reduction

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FIG. 6.
FIG. 6.

Quantification of MG-H1 and ROS in C. elegans. Formation of MG-H1 (A) and ROS (B) was quantified in 15-day-old wild-type (WT) animals, without and with 10 μmol/l myxothiazol (myxo) or 50 μmol/l FCCP, and in 15-day-old transgenic glyoxalase-1–overexpressing animals. Each group was cultured under standard and high glucose conditions. The data were compared across the groups using ANOVA; additional between-group comparisons were made using Fisher PLSD post hoc tests. Results are the means ± SE of three independent experiments with 100 nematodes each; value from a Fisher PLSD post hoc test (*P < 0.05 and ***P < 0.001) comparing the indicated group vs. wild type under standard conditions; value from a Fisher PLSD post hoc test (oooP < 0.001) comparing the indicated group vs. wild type under high glucose conditions.

This Article

  1. Diabetes vol. 58 no. 11 2450-2456