C. elegans as Model for the Study of High Glucose– Mediated Life Span Reduction

TABLE 1

Summary of individual life span experiments

Mean life span in days ± SE Max life span in days ± SE P value vs. specific group
Mean life span Maxlife span
WT (standard) 18.5 ± 0.4 25.9 ± 0.4
WT (high glucose) 16.5 ± 0.6 23.2 ± 0.4 <0.05a <0.05a
WT + sorbitol 17.9 ± 0.2 24.5 ± 0.5 n.s. (0.6438)a n.s. (0.4676)a
Glyoxalase-1 transgenic 20.3 ± 0.2 29.2 ± 1.5 <0.05a <0.05a
Glyoxalase-1 transgenic (high glucose) 20.6 ± 0.4 27.7 ± 2.3 <0.001b <0.01b
WT + glyoxalase-1 RNAi 13.5 ± 1.2 21.0 ± 1.2 <0.001a <0.01a
WT + glyoxalase-1 RNAi (high glucose) 13.9 ± 0.7 20.3 ± 1.1 <0.01b <0.05b
WT + dead bacteria 29.3 ± 0.1 40.5 ± 2.5
WT + dead bacteria (high glucose) 25.1 ± 0.1 35.5 ± 0.5 <0.01c <0.05c
eat-2 (-) mutant 24.3 ± 0.3 33.3 ± 0.3
eat-2 (-) mutant (high glucose) 21.5 ± 1.4 29.0 ± 1.5 <0.05c <0.05c
WT + daf-2 RNAi 24.2 ± 2.0 35.0 ± 3.0
WT + daf-2 RNAi (high glucose) 20.6 ± 1.7 30.0 ± 2.0 <0.05c <0.05c
WT + FCCP 21.7 ± 1.7 31.5 ± 0.5 <0.05a <0.01a
WT + FCCP (high glucose) 19.5 ± 0.9 28.5 ± 1.5 <0.05b <0.01b
WT + myxothiazol 21.1 ± 1.1 31.0 ± 1.7 <0.01a <0.01a
WT + myxothiazol (high glucose) 20.4 ± 0.1 27.7 ± 1.9 <0.001b <0.01b
  • Summary of mean and maximum (max) life span and statistical analysis for life span experiments. The data were compared across the groups using ANOVA; additional between-group comparisons were made using Fisher PLSD post hoc tests. P value from a Fisher PLSD post hoc test comparing a group vs.

  • (a) wild type (WT) under standard conditions, (b) WT under high glucose conditions, or (c) the corresponding group under standard conditions.

This Article

  1. Diabetes vol. 58 no. 11 2450-2456