Site-Specific GlcNAcylation of Human Erythrocyte Proteins

TABLE 3

O-GlcNAc site-mapping and comparison of site-specific O-GlcNAc RORs between normal and diabetic states

Protein name Accession no. O-GlcNAc peptides Peptide score Ratio D:N ROR
Spectrin-β chain, erythrocytic P11277 R.DVSSVELLMK.Y 56 1.0 0.97
K.DLTSVLILQR.K 43 1.1 1.0
K.LLTSQDVSYDEAR.N 87 1.2 1.2
R.AQGLLSAGHPEGEQIIR.L 54 1.0 0.97
R.LLSGEDVGQDEGATR.A 60 1.0 0.97
Carbonic anhydrase 1 P00915 K.YSSLAEAASK.A 58 0.20 0.40
K.ESISVSSEQLAQFR.S 86 0.29 0.57
Hemoglobin subunit-β P68871 R.FFESFGDLSTPDAVMGNPK.V 53 1.8 1.8
K.VLGAFSDGLAHLDNLK.G 75 1.2 1.2
K.GTFATLSELHCDK.L 67 1.1 1.1
Band 3 anion transport protein P02730 K.ASTPGAAAQIQEVK.E 86 0.67 0.66
K.HSHAGELEALGGVKPAVLTR.S 53 0.70 0.69
K.IPPDSEATLVLVGR.A 69 1.1 1.1
Ankyrin-1 P16157 K.LSTPPPLAEEEGLASR.I 98 1.0 0.97
K.VVTDETSFVLVSDK.H 52 1.5 1.5
R.ISEILLDHGAPIQAK.T 49 1.1 1.1
R.DSGEGDTTSLR.L 45 2.4 2.3
Spectrin-α chain P02549 R.VSSQDYGR.D 57 1.0 1.0
R.VILENIASHEPR.I 42 1.0 1.0
R.LSESHPDATEDLQR.Q 50 1.0 1.0
Peroxiredoxin-2 P32119 K.ASAVVDGAFK.E 37 0.74 0.83
R.LSEDYGVLK.T 51 0.82 0.92
Erythrocyte band 4.2 P16452 R.TQATFPISSLGDR.K 37 0.90 0.86
GLUT1 P11166 R.TFDEIASGFR.Q 59 1.1 1.3
Equilibrative nucleoside transporter 1 Q99808 K.DAQASAAPAAPLPER.N 50 1.0 1.0
Protein 4.1 P11171 R.LTSTDTIPK.S 43 1.0 1.1
Glutathione transferase ω-1 P78417 K.GSAPPGPVPEGSIR.I 56 2.5 3.2
Proteasome subunit-α type 5 P28066 K.SSLIILK.Q 54 1.3 1.4
Catalase P04040 R.LSQEDPDYGIR.D 48 1.1 1.2
R.FSTVAGESGSADTVR.D 78 2.1 3.4
α-Synuclein P37840 K.TVEGAGSIAAATGFVK.K 50 1.3 1.6
Aquaporin-1 P29972 R.SSDLTDR.V 42 1.3 1.2
Hemoglobin subunit-α P69905 K.FLASVSTVLTSK.Y 65 1.2 1.2
R.MFLSFPTTK.T 35 2.3 2.2
M.VLSPADK.T 43 1.0 0.96
  • Data are means of three experiments. Peptide scores listed are the highest scores in three independent experiments. Ratio D:N, ratio between diabetic and normal samples. ROR, O-GlcNAc ROR. Underlined residues show site of modification.

This Article

  1. Diabetes vol. 58 no. 2 309-317