TABLE 3
VH5 gene family–restricted IgA phage display libraries
| VH5 gene family IgA phage display libraries
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Type 1 diabetic TG2+ |
Type 1 diabetic TG2− |
|||||||
| Library code | N1 | N2 | N3 | N4 | N5 | N6 | N7 | |
| Library size | 3.1 × 106 | 3.2 × 106 | 1.6 × 106 | 4.1 × 106 | 6.3 × 106 | 4.6 × 106 | 2.6 × 106 | |
| Anti-TG2 antibodies | 84 | 28 | 73 | 42 | 2 | 0 | 6 | |
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Data are the percentage of positive clones. VH5 gene family–restricted IgA phage display libraries from type 1 diabetes patient lymphocytes were selected for human TG2. The library size represents the number of bacterial clones expressing an antibody fragment. After two rounds of panning, 45 clones for each library were individually tested by ELISA for TG2. Only serologically TG2+ type 1 diabetic patients have a significant number of recombinant α-human TG2 antibodies using the VH5 gene family.
