Majority of Children With Type 1 Diabetes Produce and Deposit Anti-Tissue Transglutaminase Antibodies in the Small Intestine

TABLE 3

VH5 gene family–restricted IgA phage display libraries

VH5 gene family IgA phage display libraries
Type 1 diabetic TG2+
Type 1 diabetic TG2
Library code N1 N2 N3 N4 N5 N6 N7
Library size 3.1 × 106 3.2 × 106 1.6 × 106 4.1 × 106 6.3 × 106 4.6 × 106 2.6 × 106
Anti-TG2 antibodies 84 28 73 42 2 0 6
  • Data are the percentage of positive clones. VH5 gene family–restricted IgA phage display libraries from type 1 diabetes patient lymphocytes were selected for human TG2. The library size represents the number of bacterial clones expressing an antibody fragment. After two rounds of panning, 45 clones for each library were individually tested by ELISA for TG2. Only serologically TG2+ type 1 diabetic patients have a significant number of recombinant α-human TG2 antibodies using the VH5 gene family.

This Article

  1. Diabetes vol. 58 no. 7 1578-1584