|Whole VH gene family IgA phage display libraries
|Type 1 diabetic TG2+
||Type 1 diabetic TG2−
||Control subjects without celiac disease
|Library size||8.6 × 107||8.8 × 108||3.8 × 108||7.5 × 107||6.9 × 107||1.8 × 107||2.8 × 107||5.7 × 107||5.7 × 107||6 × 107|
Data are the percentage of positive clones. IgA phage display libraries from the lymphocytes isolated from type 1 diabetes patients and control subjects without celiac disease were constructed using all of the VH gene families. The libraries were selected for human TG2 and α-gliadin. After two rounds of selection, 45 clones for each type 1 diabetes library and 90 clones for each control library were individually tested by ELISA for the corresponding antigen. All of the libraries from type 1 diabetic patients who were either serologically positive or negative for TG2 show a high percentage of positive clones.