Majority of Children With Type 1 Diabetes Produce and Deposit Anti-Tissue Transglutaminase Antibodies in the Small Intestine

TABLE 4

Whole VH gene family IgA phage display libraries

Whole VH gene family IgA phage display libraries
Type 1 diabetic TG2+
Type 1 diabetic TG2
Control subjects without celiac disease
Library code N1 N2 N3 N4 N5 N6 N7 H1 H2 H3
Library size 8.6 × 107 8.8 × 108 3.8 × 108 7.5 × 107 6.9 × 107 1.8 × 107 2.8 × 107 5.7 × 107 5.7 × 107 6 × 107
Anti-TG2 antibodies 86 88 73 46 71 68 86 10 3 0
Anti–α-gliadin antibodies 80 97 77 53 57 93 82 3 11 0
  • Data are the percentage of positive clones. IgA phage display libraries from the lymphocytes isolated from type 1 diabetes patients and control subjects without celiac disease were constructed using all of the VH gene families. The libraries were selected for human TG2 and α-gliadin. After two rounds of selection, 45 clones for each type 1 diabetes library and 90 clones for each control library were individually tested by ELISA for the corresponding antigen. All of the libraries from type 1 diabetic patients who were either serologically positive or negative for TG2 show a high percentage of positive clones.

This Article

  1. Diabetes vol. 58 no. 7 1578-1584