Whole VH gene family IgA phage display libraries
| Whole VH gene family IgA phage display libraries
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|---|---|---|---|---|---|---|---|---|---|---|
| Type 1 diabetic TG2+ |
Type 1 diabetic TG2− |
Control subjects without celiac disease
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| Library code | N1 | N2 | N3 | N4 | N5 | N6 | N7 | H1 | H2 | H3 |
| Library size | 8.6 × 107 | 8.8 × 108 | 3.8 × 108 | 7.5 × 107 | 6.9 × 107 | 1.8 × 107 | 2.8 × 107 | 5.7 × 107 | 5.7 × 107 | 6 × 107 |
| Anti-TG2 antibodies | 86 | 88 | 73 | 46 | 71 | 68 | 86 | 10 | 3 | 0 |
| Anti–α-gliadin antibodies | 80 | 97 | 77 | 53 | 57 | 93 | 82 | 3 | 11 | 0 |
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Data are the percentage of positive clones. IgA phage display libraries from the lymphocytes isolated from type 1 diabetes patients and control subjects without celiac disease were constructed using all of the VH gene families. The libraries were selected for human TG2 and α-gliadin. After two rounds of selection, 45 clones for each type 1 diabetes library and 90 clones for each control library were individually tested by ELISA for the corresponding antigen. All of the libraries from type 1 diabetic patients who were either serologically positive or negative for TG2 show a high percentage of positive clones.
