Identification of a Serum-Induced Transcriptional Signature Associated With Type 1 Diabetes in the BioBreeding Rat

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 2.
FIG. 2.

Independent analysis of genes regulated by DRlyp/lyp and DR+/+ sera. In all cases, replicates were averaged and the relatedness of the five conditions was analyzed by hierarchical clustering. A: Illustrates all probe sets regulated between the DRlyp/lyp vs. BN allogeneic inductions including probe sets of the DRlyp/lyp:DR+/+ intersection (n = 1,979). B: Illustrates all probe sets regulated between the DRlyp/lyp vs. BN allogeneic inductions excluding probe sets of the DRlyp/lyp:DR+/+ intersection (n = 1,067). Note the distinctiveness of the DRlyp/lyp signature relative to the DR+/+ and the influence of IL-1Ra in impairing induction of the DRlyp/lyp signature in panels A and B. C: Illustrates all probe sets regulated between the DR+/+ vs. BN allogeneic inductions including probe sets of the DRlyp/lyp:DR+/+ intersection (n = 2,904). D: Illustrates all probe sets regulated between the DR+/+ vs. BN allogeneic inductions excluding probe sets of the DRlyp/lyp:DR+/+ intersection (n = 1,992). Note the similarity between DRlyp/lyp and DR+/+ signatures and how the addition of IL-1Ra to DRlyp/lyp cultures enhances expression in C and D. The scale represents fold of change between the serum tested relative to autologous serum (−fourfold to +fourfold).

This Article

  1. Diabetes vol. 59 no. 10 2375-2385