Identification of a Serum-Induced Transcriptional Signature Associated With Type 1 Diabetes in the BioBreeding Rat

TABLE 2

Cytokine/chemokine levels in DRlyp/lyp, DR+/+, and BN rats

Cytokine Day 60 DRlyp/lyp Day 60 DR+/+ Day 180 BN Lower detection limit (pg/ml)
IL-1a 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 >27
IL-1b 15.6 ± 4.4 11.7 ± 6.0 11.8 ± 8.1 >27
IL-2 20.6 ± 6.9 23.8 ± 9.7 3.9 ± 3.9 >75
IL-4 1.9 ± 1.9 5.1 ± 3.9 6.7 ± 3.3 >27
IL-5 19.6 ± 10.8 0.0 ± 0.0 0.0 ± 0.0 >10
IL-6 1.6 ± 1.3 0.0 ± 0.0 1.8 ± 1.8 >250
IL-9 224.1 ± 43.9 105.0 ± 28.5 102.9 ± 55.8 >250
IL-10 107.2 ± 30.0 58.2 ± 19.4 42.7 ± 24.2 >27
IL-13 90.4 ± 21.1* 14.1 ± 2.2 14.1 ± 4.4 >27
IL-17 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 >10
IL-18 14.6 ± 3.0 12.4 ± 3.3 3.2 ± 1.6 >10
Granulocyte colony-stimulating factor 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 >10
Granulocyte/macrophage colony-stimulating factor 6.9 ± 5.2 0.0 ± 0.0 2.9 ± 2.9 >27
Growth-regulated oncogene alpha, chemokine CxCl1 302.5 ± 45.0 227.1 ± 29.9 199.8 ± 34.7 >27
Eotaxin 0.9 ± 0.9 0.0 ± 0.0 8.4 ± 2.8 >27
Interferon-γ 3.9 ± 2.3 1.6 ± 1.0 0.0 ± 0.0 >27
IP-10 0.0 ± 0.0 0.0 ± 0.0 0.2 ± 0.2 >10
Leptin 1,727.4 ± 607.1 2,478.8 ± 602.3 1,438.4 ± 203.9 >10
Monocyte chemoattractant protein (CCL2) 80.0 ± 32.9 45.8 ± 21.1 87.6 ± 7.3 >75
Macrophage inflammatory protein-1 alpha 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 >10
Rantes 1,496.9 ± 416.0 6,757.0 ± 4,493.9 1,530.2 ± 176.7 >27
TNF-α 1.6 ± 0.9 0.0 ± 0.0 16.9 ± 3.1 >10
Vascular endothelial growth factor 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 >27
IL-12p70 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 >27
IL-33 8.9 ± 0.4 6.8 ± 0.1 5.6 ± 0.5 >7
LPS§ 1.30 ± 0.2 1.34 ± 0.2 0.87 ± 0.1 >0.5
  • Data are means ± SE of six rats per group (pg/ml). Each sample was tested in duplicate using the Millipore BeadLyte cytokine assay kit, with the exception of IL-33 and LPS.

  • *P < 0.01 Wilcoxon rank-sum test vs. BN;

  • P < 0.01 Wilcoxon rank-sum test DRlyp/lyp vs. DR+/+;

  • P < 0.05 Wilcoxon rank-sum test vs. BN;

  • §conversion from endotoxin units (EU) to picograms (pg) is based upon 1 EU = 100 pg.

This Article

  1. Diabetes vol. 59 no. 10 2375-2385