Identification of a Serum-Induced Transcriptional Signature Associated With Type 1 Diabetes in the BioBreeding Rat

TABLE 3

IL-1β levels in conditioned medium after PBMC culture with DRlyp/lyp, DR+/+, and BN sera

Duration Day 60 DRlyp/lyp Day 60 DRlyp/lyp+IL-1Ra Day 60 DR+/+ Day 180 BN allogeneic Day 180 BN autologous
0 h 2.9 ± 1.7 1.7 ± 1.2 4.4 ± 2.0 2.2 ± 1.3 2.0 ± 2.0
1 h 5.3 ± 2.3 5.3 ± 2.0 4.4 ± 1.8 0.0 ± 0.0 0.7 ± 0.8
3 h 4.7 ± 2.2 7.7 ± 6.7 4.1 ± 2.3 3.3 ± 2.9 0.8 ± 0.5
6 h 3.6 ± 1.4 5.2 ± 1.9 7.7 ± 3.5 2.7 ± 2.5 1.9 ± 0.9
12 h 7.0 ± 2.5 10.3 ± 3.8 4.6 ± 1.7 9.0 ± 4.0 6.6 ± 3.3
24 h 12.4 ± 3.3* 10.1 ± 5.4 9.5 ± 4.1 1.2 ± 0.7 4.0 ± 1.8
  • Data are means ± SE of four cultures per group (pg/ml). Each culture was tested in duplicate using the IL-1β Quantikine ELISA kit (R&D Systems). In cultures possessing autologous BN sera supplemented with 1 ng/ml IL-1β, on average 816.3 ± 51.7 pg/ml was detected across the six time points. Assay sensitivity: >5 pg/ml.

  • *P < 0.05 Student t test vs. 0 h time point.

  • P < 0.05 Student t test vs. day 180 allogeneic BN sera at 24 h of culture.

This Article

  1. Diabetes vol. 59 no. 10 2375-2385