Impact of Physical Inactivity on Subcutaneous Adipose Tissue Metabolism in Healthy Young Male Offspring of Patients With Type 2 Diabetes

TABLE 5

ATGL, HSL, LPL mRNA expression relative to GADPH, and GLUT-4 mRNA expression relative to β-actin in SCAAT

CON FDR
Before BR rest (basal n = 16; insulin stimulation n = 14) After BR (basal n = 16; insulin stimulation n = 16) Before BR (basal n = 10; insulin stimulation n = 11) After BR rest (basal n = 10; insulin stimulation n = 11)
ATGL** Basal 0.10 ± 0.02* 0.10 ± 0.01 0.04 ± 0.02* 0.06 ± 0.04
ATGL** Insulin stimulation 0.12 ± 0.03 0.11 ± 0.02 0.08 ± 0.02 0.07 ± 0.01
HSL** Basal 0.10 ± 0.02* 0.15 ± 0.04* 0.02 ± 0.01* 0.01 ± 0.01*
HSL** Insulin stimulation 0.08 ± 0.01 0.09 ± 0.02 0.09 ± 0.03 0.06 ± 0.02
LPL** Basal 1.2 ± 0.2* 2.3 ± 0.4* 0.4 ± 0.1* 0.3 ± 0.2*
LPL** Insulin stimulation 1.8 ± 0.4 1.7 ± 0.2 1.0 ± 0.2 1.1 ± 0.3
GLUT 4** Basal 0.9 ± 0.3 0.6 ± 0.1 1.0 ± 0.3 1.0 ± 0.3
GLUT 4** Insulin stimulation 0.8 ± 0.2 1.2 ± 0.2 0.6 ± 0.2 2.3 ± 0.8
  • Data are mean ± SE.

  • *Significant difference CON vs. FDR, P < 0.05.

  • †Significant difference before vs. after BR within the group; P < 0.05.

  • ‡Significant difference basal vs. insulin-stimulated state within the group on the specified day, P < 0.05.

  • **Data were log transformed before statistical test.

This Article

  1. Diabetes vol. 59 no. 11 2790-2798