Recurrent Moderate Hypoglycemia Ameliorates Brain Damage and Cognitive Dysfunction Induced by Severe Hypoglycemia

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 2.
FIG. 2.

Recurrent hypoglycemia attenuates brain damage after 60 min of severe hypoglycemia. A: Blood glucose levels are shown in rats subjected to a 60-min severe hypoglycemic (10–15 mg/dl) hyperinsulinemic (0.2 units · kg−1 · min−1) clamp. Blood glucose was not significantly different between CON-SH6 (open circles) (n = 6) and RH-SH60 (closed circles) (n = 10) rats during 60 min of severe hypoglycemia. B: Representative H-E (top panel) and Fluoro-Jade B–positive (bottom panel) staining of the cortex of CON-SH60 and RH-SH60 rats 1 week following 60 min of severe hypoglycemia. Neuronal damage is indicated by pyknotic cells (H-E staining; green arrows) or with Fluoro-Jade B–positive cells (green fluorescence). Scale bar = 100 μm. C: Quantification of Fluoro-Jade B staining in CON-SH60 (white bar) (n = 6) and RH-SH60 (black bar) (n = 10) rats. Following severe hypoglycemia, RH rats had significantly fewer degenerating cells in the cortex than CON rats (*P < 0.05, by Student's t test). (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 59 no. 4 1055-1062