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Original Article

High Glucose–Induced Oxidative Stress Increases Transient Receptor Potential Channel Expression in Human Monocytes

  1. Tilo Wuensch1,2,
  2. Florian Thilo1,
  3. Katharina Krueger1,
  4. Alexandra Scholze1,
  5. Michael Ristow3 and
  6. Martin Tepel1
  1. 1Charité Campus Benjamin Franklin, Med. Klinik Nephrologie, Berlin, Germany;
  2. 2Lehrstuhl für Ernährungsphysiologie, Technische Universität, München, Germany;
  3. 3Lehrstuhl für Humanernährung, Institut für Ernährungswissenschaften Friedrich-Schiller-Universität, Jena, Germany.
  1. Corresponding author: Martin Tepel, martin.tepel{at}charite.de.
  1. T.W. and F.T. contributed equally to this study.

Diabetes 2010 Apr; 59(4): 844-849. https://doi.org/10.2337/db09-1100
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  • FIG. 1.
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    FIG. 1.

    Changes of mRNA expression of (A) TRPC3, (B) TRPC6, (C and D) TRPC1, TRPC5, TRPM6, TRPM7, and TNF-α induced by high glucose and ONOO. Exposure of monocytes to high glucose (HG; 30 mmol/l d-glucose) or ONOO (100 μmol/l) for 4 h significantly increased the TRPC3 and TRPC6 mRNA expression. The effect of high glucose is attenuated by superoxide dismutase mimetic TMP (100 μmol/l). Administration of l-glucose showed no significant effect. Control (open bar) indicates control glucose (5.6 mmol/l d-glucose). The expression of each factor is normalized to GAPDH expression. Data are mean ± SEM from at least four to six independent experiments. *P < 0.05; **P < 0.01 compared with control conditions. E: Bar graph showing TRPC3 mRNA and TRPC6 mRNA expression (normalized ratio) in monocytes from 28 age-matched control subjects (open bars) and from 18 patients with type 2 diabetes (filled bars). *P < 0.05 compared with control.

  • FIG. 2.
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    FIG. 2.

    Quantitative in-cell Western assay of TRPC3 and TRPC6 channel protein expression in monocytes treated with HG (30 mmol/l d-glucose) in the absence and presence of TMP (100 μmol/l), ONOO (100 μmol/l), l-glucose (30 mmol/l), or control (5.6 mmol/l d-glucose). A: Representative conventional Western assay (TRPC3 protein and GAPDH protein for loading control with top values indicating densitometric analysis of blots). The TRPC3/GAPDH ratio (fold over control) is also indicated. B and D: Representative in-cell Western assays and (C and E) summary data are shown. Data are means ± SEM from at least eight independent experiments. **P < 0.01 compared with control conditions.

  • FIG. 3.
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    FIG. 3.

    Recordings of fura-2 fluorescence in human monocytes. A: OAG-induced calcium influx was measured under control conditions (5.6 mmol/l d-glucose, ○) and in the presence of high glucose (30 mmol/l d-glucose) without (■) and with (▲) TMP (100 μmol/l). B: Summary data showing intracellular calcium levels after treatment of monocytes with high d-glucose for 4 h (control) stimulated with ONOO in the absence and presence of the membrane-permeable TRPC blocker, 2-APB. **P < 0.01.

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April 2010, 59(4)
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High Glucose–Induced Oxidative Stress Increases Transient Receptor Potential Channel Expression in Human Monocytes
Tilo Wuensch, Florian Thilo, Katharina Krueger, Alexandra Scholze, Michael Ristow, Martin Tepel
Diabetes Apr 2010, 59 (4) 844-849; DOI: 10.2337/db09-1100

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High Glucose–Induced Oxidative Stress Increases Transient Receptor Potential Channel Expression in Human Monocytes
Tilo Wuensch, Florian Thilo, Katharina Krueger, Alexandra Scholze, Michael Ristow, Martin Tepel
Diabetes Apr 2010, 59 (4) 844-849; DOI: 10.2337/db09-1100
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