Increased CYP2J3 Expression Reduces Insulin Resistance in Fructose-Treated Rats and db/db Mice

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FIG. 1.
FIG. 1.

Effects of CYP2J3 gene delivery on CYP2J3 protein expression, urinary 14,15-DHET levels, and fructose-induced hypertension in rats. A: CYP2J3 protein levels were increased in aorta (A), heart (H), liver (L), and kidney (K) of fructose-treated rats 2 weeks after injection of CYP2J3+ but not after injection of CYP2J3 or empty vector (pcDNA). B: Urinary 14,15-DHET levels were increased in control and fructose-treated rats injected with CYP2J3+ compared with rats injected with CYP2J3 or empty vector (pcDNA). *P < 0.01 versus pcDNA; #P < 0.01 versus CYP2J3+; n = 8 per group. C: The elevation of systolic blood pressure (BP) observed in fructose-treated rats was decreased 1, 2, and 3 weeks after injection of CYP2J3+ (i.e., at weeks 4, 5, and 6 of the study, respectively) but not after injection with CYP2J3 or empty vector (pcDNA). Values shown are mean ± SEM from each group of rats. *P < 0.01 compared with rats treated without fructose, #P < 0.01 compared with rats treated with fructose, **P < 0.01 compared with rats treated with CYP2J3+; n = 4–10 per group per time point.

This Article

  1. Diabetes vol. 59 no. 4 997-1005