Increased CYP2J3 Expression Reduces Insulin Resistance in Fructose-Treated Rats and db/db Mice

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FIG. 2.
FIG. 2.

Expression of IRS-1, eNOS, ET-1, and ETA-R in fructose-treated rats. Fructose administration to rats resulted in significantly decreased IRS-1 protein levels in liver (A) and skeletal muscle (B) and significantly decreased eNOS protein levels in aorta (C). These effects were inhibited by injection of CYP2J3+, but not by injection of CYP2J3. Representative Western blots are shown above graphs summarizing densitometric quantification. *P < 0.05 versus N + pcDNA; #P < 0.05 versus F + pcDNA; **P < 0.05 versus F + CYP2J3+; n = 8 per group. Levels of ET-1 (D) and ETA-R (E) transcripts relative to glyceraldehyde-3-phosphate dehydrogenase were assessed by RT-PCR in aortic tissue samples from three rats from each treatment group at week 5 of the study. Representative RT-PCR of ET-1 or ETA-R mRNA expression and corresponding densitometric quantification of three experiments are shown. Values shown are mean ± SEM. *P < 0.05 versus N + pcDNA3.1; #P < 0.05 versus F + pcDNA3.1; **P < 0.05 versus F + pcDNA-CYP2J3+. Effects of CYP2J3 gene delivery on rat insulin receptor signaling and eNOS expression. Fructose administration to rats resulted in significantly decreased eNOS protein levels in aorta (C) and decreased IRS-1 protein levels in liver (D) and skeletal muscle (E). These effects were inhibited by injection of CYP2J3+, but not by injection of CYP2J3. Representative Western blots are shown above graphs summarizing densitometric quantification. *P < 0.05 versus N + pcDNA; #P < 0.05 versus F + pcDNA; **P < 0.05 versus F + CYP2J3+; n = 8 per group.

This Article

  1. Diabetes vol. 59 no. 4 997-1005