Increased CYP2J3 Expression Reduces Insulin Resistance in Fructose-Treated Rats and db/db Mice

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FIG. 4.
FIG. 4.

Effects of CYP2J3 gene delivery on activation of mouse insulin receptor signaling and eNOS phosphorylation. A: Relative to levels in C57BL/6 mice, Y989-IRS-1 phosphorylation was significantly decreased and S307-IRS-1 phosphorylation significantly increased in livers of db/db normal and db/db + pcDNA mice. These changes were prevented in db/db mice injected with CYP2J3+. Representative Western blots are shown above graphs summarizing densitometric quantification. *P < 0.05 versus C57BL/6; #P < 0.05 versus db/db + pcDNA; n = 8 per group. B: Levels of pS1177-eNOS in liver were lower and levels of pT495-eNOS were higher in db/db mice than in C57BL/6 mice. The downregulation of pS1177-eNOS was inhibited by injection with CYP2J3+, whereas no effect was observed on pT495-eNOS. Representative Western blots are shown above graphs summarizing densitometric quantification. *P < 0.05 versus C57BL/6; #P < 0.05 versus db/db + pcDNA; n = 8 per group.

This Article

  1. Diabetes vol. 59 no. 4 997-1005