Increased CYP2J3 Expression Reduces Insulin Resistance in Fructose-Treated Rats and db/db Mice

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FIG. 5.
FIG. 5.

Effects of CYP2J3 gene delivery on liver PI 3-kinase (PI3K) expression in fructose-treated rats and mice. A: Decreased liver PI 3-kinase protein levels were observed in fructose-treated rats, and this was prevented by injection of CYP2J3+, but not by injection of CYP2J3 or empty vector (pcDNA). *P < 0.05 versus N + pcDNA; #P < 0.05 versus F + pcDNA; **P < 0.05 versus F + CYP2J3+; n = 8 per group. B: Liver PI 3-kinase protein levels were decreased in db/db mice compared with C57BL/6 mice. This decrease was prevented by injection of CYP2J3+ but not by injection of empty vector (pcDNA). *P < 0.05 versus C57BL/6 group; #P < 0.05 versus db/db + pcDNA; n = 8 per group. For both A and B, representative Western blots are shown above graphs summarizing densitometric quantification.

This Article

  1. Diabetes vol. 59 no. 4 997-1005