Increased CYP2J3 Expression Reduces Insulin Resistance in Fructose-Treated Rats and db/db Mice

TABLE 2

Physiological parameters determined in rats 2 weeks after injection of empty pcDNA3.1 vector, pcDNA-2J3+, or pcDNA-2J3

Variables Treatment group
Normal water treated Normal Fructose treated
N + pcDNA3.1 N + p2J3+ N + p2J3 F + pcDNA3.1 F + p2J3+ N + p2J3
Glucose (mmol/l) 5.36 ± 0.68 6.99 ± 0.06 5.69 ± 0.86 5.09 ± 0.36 6.55 ± 0.58 6.44 ± 0.68 6.85 ± 0.99
Triglycerides (mmol/l) 0.61 ± 0.22 0.63 ± 0.39 0.45 ± 0.18 0.53 ± 0.09 1.04 ± 0.89* 1.30 ± 0.13 1.26 ± 0.4
Insulin (mIU/l) 22.65 ± 0.94 18.16 ± 0.81 18.83 ± 0.44 20.5 ± 0.64 49.71 ± 0.71* 19.48 ± 0.18† 60.25 ± 0.08‡
HOMA-IR 5.26 ± 0.82 4.16 ± 0.36 6.64 ± 0.61 5.06 ± 0.26 14.25 ± 0.02* 5.40 ± 0.59† 24.28 ± 0.04‡
Body weight (g) 310 ± 34 311 ± 49 307 ± 35 299 ± 40 297 ± 49 303 ± 31 341 ± 65
  • Compared with values in the normal water–treated groups, serum insulin, insulin resistance (HOMA-IR), and serum triglycerides levels were all higher in fructose-treated rats injected with the empty pcDNA vector (F + pcDNA group). With the exception of serum triglyceride levels, all of these changes were prevented by injection of fructose-treated rats with CYP2J3+ but not CYP2J3.

  • *P < 0.05 vs. N + pcDNA3.1 group;

  • P< 0.05 vs. F + pcDNA3.1 group;

  • P < 0.05 vs. F + p2J3+ group; n = 8 per group.

This Article

  1. Diabetes vol. 59 no. 4 997-1005