Increased CYP2J3 Expression Reduces Insulin Resistance in Fructose-Treated Rats and db/db Mice

TABLE 3

Physiological parameters determined in mice 2 weeks after injection of empty pcDNA3.1 vector or pcDNA-2J3+

Variables C57BL/6 C57BL/6 + pcDNA3.1 C57BL/6 + p2J3+ db/db db/db + pcDNA3.1 db/db + p2J3+
Glucose (mmol/l) 10.48 ± 0.57 9.78 ± 0.38 9.70 ± 0.60 21.99 ± 1.83* 23.19 ± 2.51* 23.15 ± 2.36
Triglycerides (mmol/l) 1.2 ± 0.15 1.5 ± 0.22 1.43 ± 0.14 2.26 ± 0.28* 2.63 ± 0.00.32* 2.57 ± 0.37
Insulin (mIU/l) 2.06 ± 0.35 2.13 ± 0.36 1.46 ± 0.31 7.66 ± 1.33* 7.25 ± 0.87* 8.25 ± 1.96
Fasting glucose (mmol/l) 5.54 ± 0.33 5.67 ± 0.26 5.18 ± 0.40 17.52 ± 3.20* 18.80 ± 2.35* 12.65 ± 1.14†
Fasting insulin (ng/ml) 0.23 ± 0.02 0.22 ± 0.03 0.23 ± 0.01 2.49 ± 0.57* 2.618 ± 0.19* 1.43 ± 0.36†
HOMA-IR 0.053 ± 0.006 0.055 ± 0.005 0.052 ± 0.002 2.19 ± 0.72* 2.26 ± 0.45* 0.81 ± 0.24†
Body weight (g) 25.48 ± 0.61 25.45 ± 0.68 25.27 ± 0.47 45.16 ± 0.91 45.06 ± 0.97 45.78 ± 1.57
  • Serum glucose, serum insulin, insulin resistance (HOMA-IR), and serum triglyceride levels were significantly higher in the diabetic phenotype of db/db mice (injected with the empty pcDNA vector) compared with those in C57BL/6 mice. Injection of CYP2J3+ significantly reduced insulin resistance (HOMA-IR) in db/db mice, whereas injection of the empty pcDNA vector did not.

  • *P < 0.05 vs. C57BL/6 group;

  • P < 0.05 vs. db/db + pcDNA3.1 group; n = 8 per group.

This Article

  1. Diabetes vol. 59 no. 4 997-1005