Nuclear Orphan Receptor TAK1/TR4-Deficient Mice Are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance
- Hong Soon Kang1,
- Kyoko Okamoto1,
- Yong-Sik Kim1,
- Yukimasa Takeda1,
- Carl D. Bortner2,
- Huaixin Dang1,
- Taira Wada3,
- Wen Xie3,
- Xiao-Ping Yang1,
- Grace Liao1 and
- Anton M. Jetten1
- 1Cell Biology Section, Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina;
- 2Laboratory of Signal Transduction, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina;
- 3Center for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania.
- Corresponding author: Anton M. Jetten, .
OBJECTIVE The nuclear receptor TAK1/TR4/NR2C2 is expressed in several tissues that are important in the control of energy homeostasis. In this study, we investigate whether TAK1 functions as a regulator of lipid and energy homeostasis and has a role in metabolic syndrome.
RESEARCH DESIGN AND METHODS We generated TAK1-deficient (TAK1−/−) mice to study the function of TAK1 in the development of metabolic syndrome in aged mice and mice fed a high-fat diet (HFD). (Immuno)histochemical, biochemical, and gene expression profile analyses were performed to determine the effect of the loss of TAK1 expression on lipid homeostasis in liver and adipose tissues. In addition, insulin sensitivity, energy expenditure, and adipose-associated inflammation were compared in wild-type (WT) and TAK1−/− mice fed a HFD.
RESULTS TAK1-deficient (TAK1−/−) mice are resistant to the development of age- and HFD-induced metabolic syndrome. Histo- and biochemical analyses showed significantly lower hepatic triglyceride levels and reduced lipid accumulation in adipose tissue in TAK1−/− mice compared with WT mice. Gene expression profiling analysis revealed that the expression of several genes encoding proteins involved in lipid uptake and triglyceride synthesis and storage, including Cidea, Cidec, Mogat1, and CD36, was greatly decreased in the liver and primary hepatocytes of TAK1−/− mice. Restoration of TAK1 expression in TAK1−/− hepatocytes induced expression of several lipogenic genes. Moreover, TAK1−/− mice exhibited reduced infiltration of inflammatory cells and expression of inflammatory genes in white adipose tissue, and were resistant to the development of glucose intolerance and insulin resistance. TAK1−/− mice consume more oxygen and produce more carbon dioxide than WT mice, suggesting increased energy expenditure.
CONCLUSIONS Our data reveal that TAK1 plays a critical role in the regulation of energy and lipid homeostasis, and promotes the development of metabolic syndrome. TAK1 may provide a new therapeutic target in the management of obesity, diabetes, and liver steatosis.
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- Received May 4, 2010.
- Accepted September 14, 2010.
- © 2011 by the American Diabetes Association.
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