FTO Is Increased in Muscle During Type 2 Diabetes, and Its Overexpression in Myotubes Alters Insulin Signaling, Enhances Lipogenesis and ROS Production, and Induces Mitochondrial Dysfunction
- Amélie Bravard1,2,3,4,5,
- Etienne Lefai1,2,3,4,5,
- Emmanuelle Meugnier1,2,3,4,5,
- Sandra Pesenti1,2,3,4,5,
- Emmanuel Disse5,
- Julien Vouillarmet5,
- Nöel Peretti1,2,3,4,5,
- Rémi Rabasa-Lhoret6,
- Martine Laville1,2,3,4,5,
- Hubert Vidal1,2,3,4,5 and
- Jennifer Rieusset1,2,3,4,5
- 1INSERM, U-870, IFR62, Faculté de Médecine Lyon Sud, Oullins, France;
- 2INRA, UMR1235, Oullins, France;
- 3INSA-Lyon, RMND, Villeurbanne, France;
- 4Université Lyon 1, Lyon, France;
- 5Hospices Civils de Lyon, Service de Nutrition et Diabétologie, Lyon, France;
- 6Montreal Diabetes Research Center, Montreal University, Montreal, Canada.
- Corresponding author: Jennifer Rieusset, .
OBJECTIVE A strong association between genetic variants and obesity was found for the fat mass and obesity-associated gene (FTO). However, few details are known concerning the expression and function of FTO in skeletal muscle of patients with metabolic diseases.
RESEARCH DESIGN AND METHODS We investigated basal FTO expression in skeletal muscle from obese nondiabetic subjects and type 1 and type 2 diabetic patients, compared with age-matched control subjects, and its regulation in vivo by insulin, glucose, or rosiglitazone. The function of FTO was further studied in myotubes by overexpression experiments.
RESULTS We found a significant increase of FTO mRNA and protein levels in muscle from type 2 diabetic patients, whereas its expression was unchanged in obese or type 1 diabetic patients. Moreover, insulin or glucose infusion during specific clamps did not regulate FTO expression in skeletal muscle from control or type 2 diabetic patients. Interestingly, rosiglitazone treatment improved insulin sensitivity and reduced FTO expression in muscle from type 2 diabetic patients. In myotubes, adenoviral FTO overexpression increased basal protein kinase B phosphorylation, enhanced lipogenesis and oxidative stress, and reduced mitochondrial oxidative function, a cluster of metabolic defects associated with type 2 diabetes.
CONCLUSIONS This study demonstrates increased FTO expression in skeletal muscle from type 2 diabetic patients, which can be normalized by thiazolidinedione treatment. Furthermore, in vitro data support a potential implication of FTO in oxidative metabolism, lipogenesis and oxidative stress in muscle, suggesting that it could be involved in the muscle defects that characterize type 2 diabetes.
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- Received February 25, 2010.
- Accepted October 4, 2010.
- © 2011 by the American Diabetes Association.
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