Comment on: Lin et al. (2010) Immune Cell–Derived C3 Is Required for Autoimmune Diabetes Induced by Multiple Low Doses of Streptozotocin. Diabetes;59: 2247–2252

  1. Allan Flyvbjerg1
  1. 1The Medical Research Laboratories, Clinical Institute of Medicine and Medical Department of Endocrinology and Internal Medicine, NBG, Aarhus University Hospital, Aarhus, Denmark;
  2. 2Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark;
  3. 3Department of Cell Biology, Institute of Anatomy, Aarhus University, Aarhus, Denmark;
  4. 4Electron Microscopy Laboratory, Aarhus University Hospital, Aarhus, Denmark.
  1. Corresponding author: Jakob Appel Østergaard, jakob.oestergaard{at}

Lin et al. (1) report that complement factor C3 is required in mice for induction of diabetes by multiple low-dose streptozotocin injections. However, we have ourselves induced diabetes in both wild-type (WT) and C3−/− female mice on C57BL/6J background with the use of similarly sized doses of streptozotocin (Sigma-Aldrich). More than five injections were required in both groups (WT: 12 injections; C3−/−: 11 injections, P = 0.19). Mean blood glucose reached the same level (mean ± SEM 437 ± 20 mg/dl) in these groups (P = 0.21 of area under the curve [glucose × days]) (Fig. 1). After 82 days of diabetes, diabetic WT and diabetic …

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