Response to Comment on: Lin et al. (2010) Immune Cell–Derived C3 Is Required for Autoimmune Diabetes Induced by Multiple Low Doses of Streptozotocin. Diabetes;59:2247–2252
- Marvin Lin1,
- Barbara Murphy1,2,
- M. Edward Medof3,
- Stephan Segerer4,
- Peter S. Heeger1,2 and
- Bernd Schröppel1,2
- 1Division of Nephrology, Mount Sinai School of Medicine, New York, New York;
- 2Transplantation Institute, Mount Sinai School of Medicine, New York, New York;
- 3Institute of Pathology, Case Western Reserve University, Cleveland, Ohio;
- 4Division of Nephrology, University Hospital Zurich, Zurich, Switzerland.
- Corresponding author: Bernd Schröppel, .
P.S.H. and B.S. contributed equally to this letter.
The susceptibility and kinetics of diabetes induction following streptozotocin (STZ) administration is highly dependent on the specifics of the protocol (1). In our studies, we demonstrated that intraperitoneal administration of 40 mg/kg of STZ (Alexis Biochemicals, Farmingdale, NY) to male mice aged 6–10 weeks on 5 consecutive days induced autoimmune diabetes in wild-type (WT) C57BL/6 mice but not in congenic C3−/− …