Decreased IRS2 and TIMP3 Expression in Monocytes From Offspring of Type 2 Diabetic Patients Is Correlated With Insulin Resistance and Increased Intima-Media Thickness

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FIG. 2.
FIG. 2.

InsR and IRS protein levels and phosphorylation status in monocytes from lowest vs. highest insulin resistance tertiles. A–C: InsR and IRS1 protein levels and pan-tyrosine are not different in monocytes from insulin-sensitive (n = 13) vs. insulin-resistant (n = 14) subjects (A). IRS2 protein levels and pan-tyrosine phosphorylation are higher in monocytes from insulin-sensitive (n = 13) vs. insulin-resistant (n = 14) subjects (*P < 0.05, by Student t test) (B). Akt protein levels and serine 473 phosphorylation are higher in monocytes from insulin-sensitive (n = 13) vs. insulin-resistant (n = 14) subjects (*P < 0.05, by Student t test) (C). D–H: FDR subjects characterized by lowest IRS2 protein levels in monocytes exhibit significantly increased IMT (D), decreased M (E), fasting plasma glucose (F), HbA1c (G), and increased HDL (H) (n = 14 and n = 14 for LOW IRS2 and HIGH IRS2, respectively; *P < 0.05, by Student t test). a.u., arbitrary unit.

This Article

  1. Diabetes vol. 60 no. 12 3265-3270