O-GlcNAcylation Increases ChREBP Protein Content and Transcriptional Activity in the Liver

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FIG. 4.
FIG. 4.

ChREBPOG-mediated induction of the lipogenic program in the liver of OGT mice. A: Quantitative RT-PCR analysis of ACC, FAS, and SCD1. Data are means ± SEM. n = 6 per group. *P < 0.01; **P < 0.005 compared with fasted GFP mice; #P < 0.005 compared with refed GFP mice. B: Oil Red O staining of liver sections from GFP and OGT mice (original magnification ×20). C: Glucose tolerance (1 g/kg) and insulin tolerance (0.75 units/kg) tests were performed in GFP and OGT mice. Data are means ± SEM. n = 6–10 mice per group. *P < 0.01; **P < 0.005 compared with GFP mice. D: Quantitative RT-PCR analysis of G6Pase. Data are means ± SE. n = 6 per group. ##P < 0.005 compared with refed GFP mice. E: CRTC2OG were obtained by WGA binding. Specificity of the binding was confirmed by GlcNAc (0.5 mmol/L) competition. Representative Western blots (Wb) of O-GlcNAcylated and total CRTC2 forms are shown. (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 60 no. 5 1399-1413