O-GlcNAcylation Increases ChREBP Protein Content and Transcriptional Activity in the Liver

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FIG. 7.
FIG. 7.

Hepatic steatosis is improved in db/db mice overexpressing OGA. OGA (5 × 109 pfu/mouse) was overexpressed in the liver of db/db mice through penis vein injection. An equivalent dose of GFP adenovirus was injected to C57BL/6J and to another group of db/db mice. Mice were studied at the fed state. n = 5–6 mice per group. A: Quantitative RT-PCR analysis of OGA. *P < 0.05 compared with GFP mice. B: Glucose tolerance (1 g/kg) tests were performed in GFP- and OGA-treated mice. Data are means ± SEM. n = 5–6 mice per group. *P < 0.01; **P < 0.005 compared with GFP-db/db mice. C: Hepatic steatosis is prevented in OGA-db/db mice. Oil Red O staining of liver sections from GFP-C7BL/6J, GFP-db/db, and OGA-db/db mice (original magnification ×20). D: ChREBPOG and CRTC2OG levels, and ChREBP, ACC, FAS, and CRTC2 total protein content. GAPDH was used as a loading control. Representative Western blots (Wb) are shown. Lanes were run on the same gel but were noncontiguous. E: Liver and plasma TG concentrations. *P < 0.01 compared with GFP-C57BL/6J mice; #P < 0.01 compared with GFP-db/db mice. (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 60 no. 5 1399-1413