O-GlcNAcylation Increases ChREBP Protein Content and Transcriptional Activity in the Liver

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FIG. 8.
FIG. 8.

OGA overexpression in vitro leads to ChREBP deglycosylation and decreased lipid droplet accumulation. Primary mouse hepatocytes were incubated under low glucose concentrations (G5) and adeno-infected with either 5 pfu/cell of GFP or OGA adenovirus for 5 h. Cells were then incubated 24 h with high glucose concentrations plus insulin (G25i). A: Quantitative RT-PCR analysis of OGA. Data are means ± SEM. n = 4 independent cultures. *P < 0.005 compared with GFP. B: Global and specific ChREBPOG. β-Actin was used as a loading control. Representative Western blots (Wb) are shown. n = 4 independent experiments. C: Quantitative RT-PCR analysis of ChREBP, L-PK, ACC, and SCD1. *P < 0.05; **P < 0.01; ***P < 0.005 compared with GFP. Data are means ± SEM. n = 4 independent cultures. D: Oil Red O staining of hepatocytes infected with either 5 pfu/cell of GFP or OGA adenovirus (original magnification ×40). (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 60 no. 5 1399-1413