Suppression of FoxO1 Activity by Long-Chain Fatty Acyl Analogs

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 3.
FIG. 3.

Suppression of FoxO1 transcriptional trans- and coactivation by Mαα and metformin. A: Cos7 cells were transfected with FoxO1 reporter plasmid (FRE3-TK-LUC) and cotransfected with empty (–), FoxO1(TSS), or FoxO1(AAA) expression plasmids as indicated, in the presence of vehicle (black bars), 150 μmol/L Mαα (empty bars), or 2.0 mmol/L metformin (cross-hatched bars) as described in research design and methods. Luciferase activity of cells transfected with an empty plasmid normalized to β-galactosidase was defined as 1.0. Mean ± SE for three independent experiments. *Significant as compared with empty-transfected cells (P < 0.05); #significant as compared with respective vehicle-treated cells (P < 0.05). B: Cos7 cells were transfected with STAT3 reporter plasmid (M67-TATA-TK-LUC) and cotransfected with empty (–), STAT3-C, FoxO1(TSS), or FoxO1(AAA) expression plasmids as indicated, in the presence of vehicle (black bars), 150 μmol/L Mαα (empty bars), or 2.0 mmol/L metformin (dotted bars) as described in research design and methods. Luciferase activity of empty-transfected cells normalized to β-galactosidase was defined as 1.0. Mean ± SE for three independent experiments. *Significant as compared with empty-transfected cells (P < 0.05); #significant as compared with respective vehicle-treated cells (P < 0.05).

This Article

  1. Diabetes vol. 60 no. 7 1872-1881