TUNEL-positive cells increased in fixed epididymal tissue after 14 weeks of an HFD in wild-type and GM-CSF–null adipose tissue.
Adipose tissue from wild-type and GM-CSF–null mice fed an NCD or HFD for 14 weeks was fixed and subjected to TUNEL staining.
A: Dark brown nuclei are TUNEL-positive cells, and light blue is normal nuclei staining. B: Quantitated TUNEL-positive cells. Data shown are the mean ± standard error of the mean for three to six mice per group,
with 200–500 nuclei per counted per individual mouse section. Identical letters indicate values that are not statistically
different from each other (P > 0.05). C: Adipose tissue was subjected to immunofluorescence microscopy for perilipin expression using a secondary red antibody, F4/80
expression using a secondary green antibody, and nuclei by DAPI staining (blue). The white arrows indicate the cells with
loss of perilipin expression. D: Adipose tissue (from three independent mice per condition) was extracted and immunoblotted for perilipin and actin. E: Laser scanning densitometry of the immunoblots was used to quantify the ratio of perilipin expression normalized for actin.
Data shown are the mean ± standard error of the mean for four to six mice per group. Identical letters indicate values that
are not statistically different from each other (P > 0.05). WTHFD, wild-type mice fed the HFD; WTNCD, wild-type mice fed the NCD; GKOHFD, GM-CSF–null mice fed the HFD; GKONCD,
GM-CSF–null mice fed the NCD. (A high-quality digital representation of this figure is available in the online issue.)