High-Fat Diet–Induced Adipocyte Cell Death Occurs Through a Cyclophilin D Intrinsic Signaling Pathway Independent of Adipose Tissue Inflammation

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FIG. 6.
FIG. 6.

CyP-D mice are protected against HFD-induced adipocyte cell death but display glucose intolerance and insulin resistance. Wild-type and CyP-D–null 9-week-old male mice were fed an NCD or HFD for 9 weeks and then fasted for 16 h (IPGTT) or 5 h (ITT), and blood was drawn for determination of fasting glucose and insulin levels. The mice were given an intraperitoneal injection of 1 g/kg glucose (A) or 1 unit/kg insulin (B), and blood glucose and insulin levels were determined at 15, 30, 60 and 120 min. WTNCD, wild-type mice fed the NCD; WTHFD, wild-type mice fed the HFD; CKONCD, CyP-D–null mice fed the NCD; CKOHFD, CyP-D–null mice fed the HFD. Area under the curve (AUC) for each condition is shown in the insert. Data were analyzed as described in statistical analysis. Identical letters indicate values that are not statistically different from each other (P > 0.05). C: Adipose tissue extracts were prepared from wild-type and CyP-D–null mice fed an NCD or HFD as described and immunoblotted for ATGL, PDE3B, perilipin, the aP2 fatty acid binding protein, and the p115 Golgi protein as a loading control. D: Average body weight of 6 wild-type and CyP-D–null mice fed an HFD for 0, 4, and 8 weeks.

This Article

  1. Diabetes vol. 60 no. 8 2134-2143