31P-Magnetization Transfer Magnetic Resonance Spectroscopy Measurements of In Vivo Metabolism

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 2.
FIG. 2.

Direct manipulation of genes involved in mitochondrial content and metabolism modulates muscle Pi → ATP flux. Mice with muscle-specific overexpression of PGC1α (mPGC1α), a regulator of oxidative metabolism that increases mitochondrial density and enhances expression and protein content of genes involved in oxidative phosphorylation, exhibit a concomitant increase in Pi → ATP flux compared with wild-type (WT) mice. Muscle Pi → ATP flux is decreased in transgenic mice that overexpress UCP3 (UCP3-TG, D.E.B. and G.I.S unpublished observations), a mitochondrial membrane protein found in skeletal muscle that may uncouple the H+ electrochemical gradient across the inner mitochondrial membrane and decrease the efficiency of oxidative phosphorylation. Data obtained from UCP3-TG mice were acquired using the same experimental protocols described in Choi et al. (22). Data adapted from Choi et al. (22), reproduced courtesy of the National Academy of Sciences.

This Article

  1. Diabetes vol. 61 no. 11 2669-2678