Vaspin Is an Adipokine Ameliorating ER Stress in Obesity as a Ligand for Cell-Surface GRP78/MTJ-1 Complex
- Atsuko Nakatsuka1,
- Jun Wada1⇓,
- Izumi Iseda1,
- Sanae Teshigawara1,
- Kanji Higashio3,
- Kazutoshi Murakami1,
- Motoko Kanzaki1,
- Kentaro Inoue1,
- Takahiro Terami1,
- Akihiro Katayama1,
- Kazuyuki Hida1,
- Jun Eguchi1,
- Chikage Sato Horiguchi2,
- Daisuke Ogawa2,
- Yasushi Matsuki4,
- Ryuji Hiramatsu4,
- Hideo Yagita5,
- Shigeru Kakuta6,
- Yoichiro Iwakura6,7 and
- Hirofumi Makino1
- 1Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
- 2Department of Diabetic Nephropathy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
- 3Metabolome Pharmaceuticals, Inc., Tokyo, Japan
- 4Genomic Science Laboratories, Dainippon Sumitomo Pharma, Osaka, Japan
- 5Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan
- 6Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
- 7Core Research for Evolutional Science and Technology, Japan Science and Technology, Saitama, Japan
- Corresponding author: Jun Wada, .
It is unknown whether adipokines derived from adipose tissues modulate endoplasmic reticulum (ER) stress induced in obesity. Here, we show that visceral adipose tissue–derived serine protease inhibitor (vaspin) binds to cell-surface 78-kDa glucose-regulated protein (GRP78), which is recruited from ER to plasma membrane under ER stress. Vaspin transgenic mice were protected from diet-induced obesity, glucose intolerance, and hepatic steatosis, while vaspin-deficient mice developed glucose intolerance associated with upregulation of ER stress markers. With tandem affinity tag purification using HepG2 cells, we identified GRP78 as an interacting molecule. The complex formation of vaspin, GRP78, and murine tumor cell DnaJ-like protein 1 (MTJ-1) (DnaJ homolog, subfamily C, member 1) on plasma membrane was confirmed by cell-surface labeling with biotin and immunoprecipitation in liver tissues and H-4-II-E-C3 cells. The addition of recombinant human vaspin in the cultured H-4-II-E-C3 cells also increased the phosphorylation of Akt and AMP-activated protein kinase (AMPK) in a dose-dependent manner, and anti-GRP78 antibodies completely abrogated the vaspin-induced upregulation of pAkt and pAMPK. Vaspin is a novel ligand for cell-surface GRP78/MTJ-1 complex, and its subsequent signals exert beneficial effects on ER stress–induced metabolic dysfunctions.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db12-0232/-/DC1.
- Received February 23, 2012.
- Accepted May 26, 2012.
- © 2012 by the American Diabetes Association.
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