The Magnetic Appeal of Silencing Theranostics
Islet cell transplantation has recently emerged as a promising means to treat patients with type 1 diabetes. Despite significant success with the Edmonton protocol for intraportal naked islet transplantation (1), there is an active debate regarding whether or not the overall clinical success rates have met initial expectations (2,3). The next vital steps for improving islet cell transplantation protocols include developing a nontoxic and effective means to prevent graft rejection and islet cell death, as well as suitable imaging techniques to noninvasively probe islet engraftment and long-term survival.
Although it is currently unknown how long and what percentage of grafted cells survive, estimates suggest that up to only 30% of the initial β-cell mass remains after 2 weeks (4). It is believed that, aside from immunorejection, rapid islet cell death may occur from lack of oxygen before and after transplantation. One way to increase islet cell survival is to store them in perfluorocarbon emulsions that can act as an oxygen sink, leading to improved oxygenation (5). Another approach is to prevent immunorejection by encapsulating islets in semipermeable alginate capsules (6) or a combination of the two approaches (7). Although monitoring of islet engraftment following intraportal injection is possible by prelabeling naked cells (8,9) or encapsulated cells (10) with a superparamagnetic iron oxide (SPIO) contrast agent followed by magnetic resonance imaging (MRI), imaging of islet survival has been much more challenging (11).
In this issue of Diabetes, Wang et al. (12) have used an elegant and clever approach for enhancing graft survival by prelabeling them with a novel theranostic nanoprobe. The formulation consists of small interference RNA (siRNA) targeting β2-microglobulin (B2M), the fluorescent dye Cy5.5 for optical detection, and SPIO nanoparticles for MRI detection (Fig. 1). The rationale for silencing B2M is to knock down functional expression of the major histocompatibility complex class I, of which B2M is a major component. A dysfunctional major histocompatibility complex class I expression will result in an impaired adaptive immune response by cytotoxic CD8+ T cells, one of the major factors driving islet immunorejection and graft failure (13,14). Cy 5.5 was incorporated primarily to assess the efficacy of B2M knockdown in vitro using fluorescent microscopy, and SPIO particles were used to evaluate graft survival in vivo through loss of signal as a surrogate marker (upon cell death, the magnetic nanoparticles are released and rapidly metabolized by host macrophages).
Freshly isolated cadaveric human islets were prelabeled by incubation with the theranostic targeted at silencing B2M (25 μg Fe/mL, 1.8 pmol siRNA/μg Fe) for 48 h in culture. These islets were then injected under the kidney capsule of immunodeficient B2M-deficient NOD/scid mice that lacked endogenous CD8+ T cells and that were made diabetic by intraperitoneal injection of streptozotocin, and then, at 1 week posttransplantation, splenocytes from immunocompetent NOD mice were adoptively transferred to compare the immunorejection and survival of islets with and without prelabeling of the theranostic probe (control particles contained scrambled siRNA in lieu of B2M siRNA). It was demonstrated that prelabeling with either probe did not result in loss of viability before transplantation. The B2M mRNA expression was reduced by 46% following incubation, with a concomitant decrease in protein expression as detected by Western blot and immunohistochemistry and a reduced number of activated CD8+ T cells in vitro.
In vivo, the hypointense MRI signals from the magnetic theranostic persisted longer when the islets were pretreated with the siRNA B2M probe. This correlated to a significant delay, up to 24 days in diabetes onset after the adoptive T-cell transfer as compared with 7 days for the controls. Prelabeling islets with the B2M-silencing theranostic probe was accompanied by a lower infiltration of CD8+ T cells, presumably as a result of the knockdown of B2M expression. Thus, the study by Wang et al. (12) has shown a proof-of-principle that xenografted islets can be protected from immediate immunorejection by simple prelabeling with an siRNA-based theranostic. It remains to be seen, however, if their approach will result in long-term islet protection. All grafts in this study eventually failed after 30 days posttransplantation, indicating that further therapeutic refinement will be needed. It is possible that knocking down a response by the adaptive immune system (CD8+ T cells) may be further enhanced by silencing ligands involved in binding of cells belonging to the innate immune system (e.g., natural killer cells, which are also involved in islet allograft rejection) (14).
Nonetheless, a noninvasive imaging technique to evaluate a long-term successful outcome of siRNA silencing that can be applied in patients, if successfully translated, is still not available at the present time. In preclinical studies, endowing islet cells with an optical gene allows short-term monitoring of islet cell survival (15), but this approach is not clinically translatable. Unfortunately, the indirect approach by Wang et al. (12) to endow the theranostic probe with magnetic properties to monitor graft volume in vivo may not be straightforward. Clinically, it has been shown that the number of hypointense spots on MRI does not correspond to the number of islets that were injected (16), and therefore, other techniques will need to be developed to noninvasively determine the efficacy and outcome of RNA silencing and immunomodulation.
No potential conflicts of interest relevant to this article were reported.
See accompanying original article, p. 3247.
- © 2012 by the American Diabetes Association.
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