Disruption of Growth Factor Receptor–Binding Protein 10 in the Pancreas Enhances β-Cell Proliferation and Protects Mice From Streptozotocin-Induced β-Cell Apoptosis

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FIG. 3.
FIG. 3.

Pancreatic knockout of Grb10 increases glucose tolerance and insulin secretion. A: GTT was performed in 5-month-old male pGrb10KO mice (n = 9) and age-matched WT littermates (n = 8) fed with normal diet. Blood glucose was withdrawn from the tail vein at 0, 15, 30, 60, and 120 min after intraperitoneal injection of glucose (2 g/kg body weight) and measured using a glucometer. B: GTT was performed on overnight-fasted male pGrb10KO mice and WT littermates (WT: n = 11; pGrb10KO: n = 9) fed with 60% HFD for 16 weeks. Blood glucose after intraperitoneal injection of glucose (2 g/kg body weight) was withdrawn from the tail vein at 0, 15, 30, 60, and 120 min. C: The glucose infusion rates in 3-month-old normal chow diet male pGrb10KO and WT control mice were determined by hyperglycemic clamp experiments (n = 4/group). D: Insulin levels of male pGrb10KO and WT littermates during hyperglycemic clamp experiments (n = 4/group). The data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 (one-way repeated-measure ANOVA).

This Article

  1. Diabetes vol. 61 no. 12 3189-3198