CGI-58 KD alters the systemic inflammatory and metabolic response to endotoxin. C57BL/6N mice were fed a standard chow diet
in conjunction with biweekly injections of a nontargeting control ASO (□) or CGI-58 ASO (■) for 4 weeks. Thereafter, mice
received a single intraperitoneal injection of either saline or LPS (5 μg/mouse) and were necropsied 6 h after injection.
A: Plasma cytokine and acute-phase response protein levels were measured at 1 h (for TNFα only) and 6 h after injection (for
IL-6 and IL-12p40 and for SAA and haptoglobin). ND, levels below limit of detection. B and C: qPCR analyses of hepatic (B) and epididymal adipose tissue (C) gene expression. SAP, serum amyloid P-component. WAT, white adipose tissue. D: Hepatic TAG levels. E: Plasma TAG levels at 6 h after injection. Data in A, D, and E represent the mean ± SEM (n = 6), and qPCR data in B and C represent the mean ± SEM (n = 5). Within each panel, values not sharing a common superscript letter differ significantly (P < 0.05). AU, arbitrary unit.