Liver tissue analysis after food enrichment with MUFAs or SFAs. A and B: Attenuated phosphorylation of IR and AKT in liver tissue of SFA-fed animals. Liver lysates were separated by SDS-PAGE, and
tyrosine phosphorylation (PY) of IR (A) and AKT (S473) (B) as well as protein expression of IR and AKT was analyzed after intravenous insulin injection (cross-hatched bars) in overnight-fasted
MUFA-, SFA-, or chow-fed animals. Densitometric quantification of PY-IR (relative increase of PY-IR compared with insulin-stimulated
control mice; n = 6–8/group; ***P < 0.001) is presented in histograms. Ins, insulin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.