Genetic and environmental impact on number (#) and percentage (%) of APCs and other SVF cells from Vis and SC fat depots.
FACS sorting analysis of Vis and SC APCs and other SVF cells. Data were calculated using only live cells. Double positive
CD34, SCA1 (and CD45−, CD31−, and Ter119−) cells were considered APCs, whereas CD45+, CD31+, and Ter119+ cells were considered as other SVF cells. A: 8-week-old vs. 1-year-old C57BL/6 mice. m, month. B: C57BL/6 (B6) vs. 129 mice (3 months old). C: C57BL/6 male (M) vs. female (F) mice (6 months old). Five mice per group were used in two independent experiments. Data
are mean ± SEM. Statistics were analyzed by Student t test (two-way, unequal variance). *P < 0.05.