Effect of HFD on number (#) and percentage (%) of APCs and other SVF cells from Vis and SC fat depots. FACS sorting analysis
of Vis and SC APCs and other SVF cells. Data were calculated using only live cells. Double positive CD34, SCA1 (and CD45−, CD31−, and Ter119−) cells were considered APCs, whereas CD45+, CD31+, and Ter119+ cells were considered as other SVF cells. FACS sorted cells from mice placed on chow diet (CD) or HFD for 1, 2, and 4 weeks
starting at age 4 weeks (A) or 8 weeks (B). Three to five mice per group were used in two independent experiments. Data are mean ± SEM. Statistics were analyzed by
Student t test (two-way, unequal variance). *P < 0.05.